Coding

Part:BBa_K1342003:Design

Designed by: Naoki Saito,Daiki Haraguchi,Yoshiharu Otaki,Ryuhei Minei   Group: iGEM14_Biwako_Nagahama   (2014-10-03)


SmtA Part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 121
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

One silent mutation was introduced to eliminate PstI restriction enzyme site and was eliminated at bases 96 with C → T mutation.

Source

Synechococcus sp. PCC7942

References

Construction of a marine cyanobacterial strain with increased heavy metal ion tolerance by introducing exogenic metallothionein gene, Koji Sode et al., J Mar Biotechnol (1998), 6:174-177